An In Vitro Procedure for Phenotypic Screening of Growth Parameters and Symbiotic Performances in Lotus corniculatus Cultivars Maintained in Different Nutritional Conditions
نویسندگان
چکیده
The establishment of legumes crops with phenotypic traits that favour their persistence and competitiveness in mixed swards is a pressing task in sustainable agriculture. However, to fully exploit the potential benefits of introducing pasture-based grass-legume systems, an increased scientific knowledge of legume agronomy for screening of favourable traits is needed. We exploited a short-cut phenotypic screening as a preliminary step to characterize the growth capacity of three different Lotus corniculatus cvs cultivated in different nutritional conditions as well as the evaluation of their nodulation capacities. This experimental scheme, developed for legume species amenable to grow on agar plates conditions, may represent a very preliminary step to achieve phenotypic discrimination on different cultivars.
منابع مشابه
Growth and physiological parameters under salinity stress in Lotus corniculatus. Marjan Azarafshan and Nasser Abbaspour *
Salinity is a main and common stress which decreases the amount of agriculture products and natural plants in many areas of the world. In this study, the effect of salt stress on three varieties of Lotus corniculatus 'Karaj', 'Jolfa', and 'Ardabil' was investigated. Plants were cultured in hydroponic condition with four NaCl treatments (0, 50, 100, 150 mM). The amounts of proline, glycine betai...
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In order to understand the response of Lotus corniculatus to salt stress, ions content and some growth factors were analyzed in three varieties of this plant, namely, Ardebil, Karaj, and Jolfa. Lotus corniculatus plants were exposed to 0, 50, 100, and 150 mM NaCl in hydroponic condition. The amounts of anions (Cl-and NO3-) and cations (K+ and Na+) in leaves as well as shoot and root dry weights...
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Lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. The aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with Lotus spp. A total of 180 isolates able to nodulate Lotus cornicul...
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Enlarged root systems that extend into the salt affected soil improve water and nutrient capture by plants and can increase plant productivity. In order to examine root system characteristics of four bread wheat cultivars contrasting in salt tolerance (Arg, Ofoq, Tajan and Morvarid) a greenhouse experiment was conducted with applying two salinity levels (0 and 150 mM NaCl) on plants grown in PV...
متن کاملDe novo alanine synthesis by bacteroids of Mesorhizobium loti is not required for nitrogen transfer in the determinate nodules of Lotus corniculatus.
Deletion of both alanine dehydrogenase genes (aldA) in Mesorhizobium loti resulted in the loss of AldA enzyme activity from cultured bacteria and bacteroids but had no effect on the symbiotic performance of Lotus corniculatus plants. Thus, neither indeterminate pea nodules nor determinate L. corniculatus nodules export alanine as the sole nitrogen secretion product.
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